Plasmodium falciparum Pf34, a novel GPI-anchored rhoptry protein found in detergent-resistant microdomains
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چکیده
منابع مشابه
Trafficking of Plasmodium falciparum chimeric rhoptry protein with Brefeldin A.
Trafficking of the rhoptry chimeric protein RhopH2-GFP, which contains RhopH2 signal peptide plus the downstream five amino acids, was dissected by treating parasites with Brefeldin A at three different time points. Twenty eight hrs-stage trophozoites accumulated the chimera within the parasite endoplasmic reticulum. In 32 hrs-stage schizonts, the chimera was distributed in the parasite cytopla...
متن کاملCharacterization of a membrane-associated rhoptry protein of Plasmodium falciparum.
Invasive forms of apicomplexan parasites contain secretory organelles called rhoptries that are essential for entry into host cells. We present a detailed characterization of an unusual rhoptry protein of the human malaria parasite Plasmodium falciparum, the rhoptry-associated membrane antigen (RAMA) that appears to have roles in both rhoptry biogenesis and host cell invasion. RAMA is synthesiz...
متن کاملConserved high activity binding peptides from the Plasmodium falciparum Pf34 rhoptry protein inhibit merozoites in vitro invasion of red blood cells.
Rhoptries are specialized secretory organelles found in all members of the genus Plasmodium whose proteins have been considered as promising vaccine candidates due to their involvement in cell invasion and the formation of the parasitophorous vacuole (PV). The Plasmodium falciparum Pf34 protein was recently identified as a rhoptry-neck protein located in detergent-resistant microdomains (DRMs) ...
متن کاملMicrodomains of GPI-anchored proteins in living cells
| There is some discussion as to whether glycosyl-phosphatidylinositol(GPI)-anchored proteins occur in microdomains in the cell membrane. These putative microdomains have been implicated in processes such as sorting in polarized cells and signal transduction. Complexes enriched in GPI-anchored proteins, cholesterol and glycosphingolipids have been isolated from cell membranes by using non-ionic...
متن کاملA role for adaptor protein complex 1 in protein targeting to rhoptry organelles in Plasmodium falciparum.
The human malaria parasite Plasmodium falciparum possesses sophisticated systems of protein secretion to modulate host cell invasion and remodeling. In the present study, we provide insights into the function of the AP-1 complex in P. falciparum. We utilized GFP fusion constructs for live cell imaging, as well as fixed parasites in immunofluorescence analysis, to study adaptor protein mu1 (Pfμ1...
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ژورنال
عنوان ژورنال: International Journal for Parasitology
سال: 2007
ISSN: 0020-7519
DOI: 10.1016/j.ijpara.2007.03.013